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X-WR-CALNAME:Department of Electrical and Computer Engineering (HKUECE) 電機與計算機工程系
X-ORIGINAL-URL:https://ece.hku.hk
X-WR-CALDESC:Events for Department of Electrical and Computer Engineering (HKUECE) 電機與計算機工程系
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TZID:Asia/Hong_Kong
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TZOFFSETFROM:+0800
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DTSTART:20230101T000000
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DTSTART;TZID=Asia/Hong_Kong:20241018T140000
DTEND;TZID=Asia/Hong_Kong:20241018T150000
DTSTAMP:20260512T125842
CREATED:20241010T015619Z
LAST-MODIFIED:20250114T034021Z
UID:19303-1729260000-1729263600@ece.hku.hk
SUMMARY:Enhancing Contrast\, Field of View and Axial Resolution of Structured Illumination Microscopy
DESCRIPTION:Direction to Innovation Wing Two: https://innowings.engg.hku.hk/innowing2/visitors \nAbstract\nSuper-resolved structured illumination microscopy (SR-SIM) is among the most flexible\, fastest and least perturbing fluorescence microscopy techniques capable of surpassing the optical diffraction limit. Current custom-built instruments are easily able to deliver two-fold resolution enhancement at video-rate frame rates\, but the cost of the instruments is still relatively high and the physical size of the instruments is still prohibitively large. We have developed compact\, cost-efficient and high-speed 2D-\, grazing-incidence- and TIRF-SIM to overcome these limitations. A fiber-based illumination path allows multi-color imaging with a field-of-view (FOV) of up to 230×230 µm². A novel method called multi-angle TIRF SIM allows an image reconstruction with an enhanced axial resolution. The setup consists of a fiber-coupled laser combiner with 405 nm\, 491 nm\, 561 nm and 639 nm laser sources\, a fiber-switch to select the desired illumination angle with integrated phase shifters and a hexagonal fiber collimator with a lens telescope that projects the beams into the back-focal plane of the objective lens. All modules of the illumination path are home-built to achieve highest stability and a compact size. The rotation of the illumination angle is performed by two galvo-mirrors that select the corresponding pair of fibers and phase shifts of the zero order beam and one of the two first order beams are performed by home-built\, MEMS-mirror based phase-shifters that modulate the optical path length. The phase shifters and galvo-mirrors allow transition times between illumination patterns of less than 1 ms. The hexagonal collimator allows to adjust the pattern spacing in the sample seamlessly\, within a transition time of 100 ms. The capability of the setup is demonstrated by multicolor imaging of fixed liver endothelial cells (LSECs) and mouse brain tissue slices with TIRF- and 2D-SIM with a FOV of up to 230×230 µm² and a lateral resolution of up to 85 nm. Moreover\, an improved version of this setup was used with a wide-field FLIM detector for super-resolved FLIM-SIM\, which was applied for live-cell imaging and dye-multiplexing. \nSpeakers: Dr. Henning Ortkrass & Mr. Manuel Kunisch\nBielefeld University \nBiography of the Speakers: \nHenning Ortkrass is PostDoc in the research group of Prof. Dr. Thomas Huser in the field of biomolecular photonics. He obtained his PhD in 2024 with a thesis on novel approaches on super-resolution structured illumination microscopy (SIM)\, including the development of a large-FOV\, multi-color\, high-speed\, fiber-based TIRF-SIM system. Additionally\, he developed and applied a compact\, cost-efficient\, interferometric SIM system for joint fluorescent lifetime imaging microscopy. These systems were successfully used in live-cell applications. Besides his research focus on novel soft- and hardware solutions for more versatile SIM microscopy\, he currently prepares a start-up company to make this methods more user-friendly and commercially available. \nManuel Kunisch is PhD student at the Biomolecuar Photonics lab led by Thomas Huser at the Bielefeld University. He obtained his Bachelor’s degree in 2021 with a thesis about the fast reconstruction of hyperspectral coherent Raman scattering (HS-CRS) data. In this context\, a Python-based reconstruction tool with a user-friendly GUI was conceived and is still under development to facilitate the creation of false-color coded images from hyperspectral data separating distinct molecular groups based on their spectral response in individual channels. \nOrganiser: Professor Kenneth K.Y. Wong\nDepartment of Electrical and Electronic Engineering\nThe University of Hong Kong \nCo-organiser: Tam Wing Fan Innovation Wing Two \nAll are welcome! We look forward to seeing you!
URL:https://ece.hku.hk/events/20241018-1/
LOCATION:Tam Wing Fan Innovation Wing Two\, G/F\, Run Run Shaw Building\, The University of Hong Kong
CATEGORIES:Seminar
ATTACH;FMTTYPE=image/jpeg:https://ece.hku.hk/wp-content/uploads/2024/10/1280-4.jpg
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